Recombinant PNGase F，

LudgerZyme recombinant PNGase F kit (New England BioLabs).

PNGase F is suitable for the release of all types (high-mannose, hybrid and complex) N-linked glycans from glycoproteins and glycopeptides. PNGase F will not remove oligosaccharides containing α(1-3) linked core fucose commonly found on plant glycoproteins.

LudgerZyme PNGase F is a recombinant glycosidase cloned from Elizabethkingia miricola and expressed in E. coli. The enzyme is supplied glycerol-free (for optimal performance in HPLC-intensive methods) along with Reaction Buffer, Denaturation Solution and NP-40 Solution for efficient de-glycosylation.

LudgerZyme Peptide N-glycosidase F (PNGase F) is suitable for the release of N-linked glycans in solution and from immobilized samples. The enzyme cleaves between the innermost GlcNAc of the oligosaccharide moiety at its attachment point to the asparagine residue on the protein and subsequently converts the asparagine into aspartic acid. Released glycans with free reducing terminus can be labelled using LudgerTag labelling technology for fluorescence and high MS sensitivity detection.

Kit includes enzyme plus reaction buffers. Sufficient for up to 150 reactions.

Note:  The LZ-rPNGaseF-30 enzyme kit part of the LT-KAB-VP30-MOD contains enzyme plus reaction buffers that is sufficient for 30 reactions.

https://www.ludger.com/product-catalogue/recombinant-pngase-f/