2AB标记的多糖标准品溶解方法：

我们2AB标记的多糖是粉末状态，量比较少，差不多只有100pmol. 所以很多时候是看不出来的，这很正常。

CAB-XX-01 可以溶解到50ul 的水中，UPHLC分析上样用5ul 即可。一次使用不完可以存放-20℃，再次复溶。

https://www.ludger.com/product-catalogue/standards-controls/n-glycan-2-ab-labelled

未标记的多糖标准品溶解方法：

CN-XXX-10U标准品溶解于200uL水中，然后取 0.5uL用于MALDI分析。只需直接加水到小瓶中并漩涡10秒，确保水沿着管子内壁流动，混合均匀。

For the CN-XXX-10U, I confirm it is dissolved in 200uL water and then use 0.5uL of it for MALDI. Just add water directly to the vial and vortex for 10 sec, 

making sure that water is going around the inner wall of the tube. 

The CN- glycan standard is a single species glycan. 产品信息，点击链接：https://www.ludger.com/product-catalogue/standards-controls/n-glycan-unlabelled。

The CLIBN-IGG consists of polyclonal IgG and thus has a high number of glycoforms and a high number of glycopeptides species. 

This means that the signal is diluted by having this larger range of glycopeptide structures, each of which is present at a lower level than if the sample had been a single glycoform.

That is why they couldn’t see any signal from the CLIBN-IGG sample and they should get signal from the single glycoform standard. 

Another reason is the CLIBN-IGG they bought might be the 5ug aliquot not 25ug (see CofA)