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procainamideGlycanLabelingKit的敏感性原理：
MS的信号增强，主要是在普鲁卡因的末端增加一个季胺基团，季胺基在ESI下很容易带上正电荷，因此质谱正离心模式下信号得道显著增强，比2-AB增强N倍，解决了广大用户在FLR上信号理想，但MS信号较弱，无法定性的困扰.
The achievement of high sensitivity of the procainamide labelled glycans in the positive ion mode can be attributed to the high proton affinity of the basic tail, a 2-(diethylamino)ethyl group of the procainamide.
The labelling efficiency have been evaluated and tested by Ludger Scientists and the reaction conditions are altered to provide high labelling efficiency.
普鲁卡因胺标记允许用质谱或UHPLC 识别寡糖,正因为与2 ab标记相比它的电离作用效率提高,它可以允许用ESI-MS识别小聚糖组分(> 1%的相对峰面积)。我们很高兴地宣布推出一个Ludger用普鲁卡因胺标记多糖的试剂(使用----2-甲基吡啶硼烷(2-PB)作为还原剂代替氰基氢硼化钠),包含一个样品标记后的纯化板。这项技术已经在Ludger实验室经过验证，而且三次平行分析的显著误差＜5%
相比2 AB、普鲁卡因胺标记具有以下特点:
•与(U)HPLC和MS兼容；
•提高低丰度寡糖的鉴定；
•提高质谱离子化效率；
•使得用二级质谱（MS/MS）碎裂测定低丰度的寡糖组分的结构成为可能；
•适合于用LC-MS分析普鲁卡因胺标记的中性和唾液酸化多糖(使用LudgerTag普鲁卡因胺标记试剂盒，货号# LT-KPROC-24)，它是一个可用于多糖的相对定性和定量分析的单一体系。它可用于以下:
1.生物药物，如：促红细胞生成素，IgG等；
2.生物样品，如唾液酸；
3. O-glycan分析。
 每个试剂盒有两套试剂，每套有3个小瓶。Each kit contains two sets of three vials每一个set可以做12个样品，总共可以做24个样品。从样品处理开始，整个分析过程只需要1.5小时
Procainamide labelling permits glycan identification by either mass spectrometry or UHPLC, and because of its improved ionisation efficiency compared to 2AB labelling it can permit identification of minor glycans (>1% relative peak area) by ESI-MS. We are delighted to announce the launch of a Ludger kit for labelling glycans with procainamide (using 2PB as a reductant in place of sodium cyanoborohydride), along with a post-labelling clean up plate for the samples. This technology has been validated in house at Ludger; typical CVs for triplicate analyses were <5%. Comparability of data for 2AB and procainamide labelled IgG analysed by HILIC-UPLC:
(更多试剂盒详情，请点击下载http://www.ludgersh.com/9ysh/up/updown/20151439215781.pdf ）
Procainamide labelling ktis 操作使用操作步骤（图片展示）
http://www.ludgersh.com/9ysh/up/updown/20153554808777.pdf |
