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LudgerTag V-Tag™ for Glycopeptide Profiling 糖肽分析：Ludger V-tag可以直接对肽段进行分析
更详细内容可以点击链接：http://www.ludgersh.com/9ysh/up/updown/20157639439981.pdf
A validated system for labeling glycopeptides from IgG mAbs enabling analysis by U-HPLC and/or MALDI-MS.
• Glycopeptides are labeled with V-Tag dye and enriched to enhance signal intensity on MALDI-MS
• Minimal Sample needed; as little as 10µg of glycoprotein
• Labeling and enrichment is completed within 2 hours
• GMP compatible: Validated according to ICH Q2 standards.
V-Tag kit是一个经过验证的针对IgG的糖肽的标记和富集试剂盒，通过（U）HPLC或MALDI质谱分析分析。
•糖肽用V-Tag染料标记并且富集以提高在MALDI-MS上的信号敏感度。
•仅仅需要10μg的IgG样品都可以进行检测。
•VTAG试剂盒可以在2小时内完成每个样品的标记和使用固相萃取（SPE）装置富集样品。
•GMP相容性：该试剂盒已经是根据ICH指南Q2（R1）验证了的，使用不同的IgG样品和每个样品重复9次，可重复性误差更是明显<5％。
•V-TAG试剂盒（目录号LT-VTAG-24）适合于用链霉蛋白酶或胰蛋白酶释放的糖肽IgG亚类的糖蛋白。
LudgerMass Service LudgerMass-MALDI is a new service that will be launched in Autumn 2015 to give you access to mass spectra of your sample. - Fixed price per sample - Appropriately prepared samples* accepted for analysis - A range of matrices can be selected.
Setting up sialic acid analysis in-house Sialic acid analysis is a regulatory requirement laid out in the ICH Q6B guidelines for characterisation of biopharmaceuticals. The LudgerTag™ DMB kit provides all that is required to release sialic acids from glycoproteins and label them with DMB prior to analysis by (U)HPLC. For positive controls, we recommend using fetuin glycoprotein which contains both Neu5Ac and Neu5Gc sialic acids. We also recommend a purified sialylated glycopeptide standard, the first in a range of Ludger BioQuant™ quantitative standards, as a positive control. This standard (Cat No. BQ-GPEP-A2G2S2-10U) is a complex biantennary N-linked glycan terminating in two N-acetylneuraminic acids. Using this standard will enable you to check the efficiency of glycan release, labeling and recovery and will give you confidence in the accuracy of your sialic acid measurements. Ludger also sells a standard which can be used for identification of Neu5,9Ac2 which is present in human erythropoietin (EPO). Formulation buffer can be used as a negative control. Ludger Products Cat.No. Release and label sialic acids with DMB: LudgerTag™ DMB Sialic Acid Labelling Kit LT-KDMB-A1 Sialylated Positive Controls: Fetuin Glycoprotein (4 x 50 ug) GCP-FET-50U-X4 Quantitative Glycopeptide Standard BQ-GPEP-A2G2S2-10U Neu5,9Ac2 standard CM-NEU5,9,AC2-01 N-acetylneuraminic acid quantitative standard CM-NEU-AC-01 N-glycolyneuraminic acid quantitative standard CM-NEU-GC-01 HPLC analysis: LudgerSep™ R1 HPLC Column LS-R1-4.6x150 U-HPLC analysis: LudgerSep™ uR2 UHPLC Column LS-UR2-2.1x100
我们为中国客户提供一流的产品售后服务及相应的方法技术支持！同时提供糖基化系列技术服务（技术转移到中国或者样品送到国外进行分析），如您有需要请及时跟我们联系！
中国办事处联系方式：
王丽 (Lily) 李慧 (Cindy)
Mobile: +86 186 0210 8329 Mobile: +86 157 1167 5909
Email: lily.wang@ludger.com Email: cindy.li@ludger.com
QQ: 258 363 672 QQ: 170 439 096
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