The kit contains reagents for the release of N and O-linked glycans from glycoproteins via hydrazinolysis.
The LudgerLiberate Hydrazinolysis Kit contains reagents for the release of N and O-linked glycans from glycoprotein biopharmaceuticals. Released glycans have free-reducing terminii to allow fluorescent tagging by reductive amination. The release conditions can be optimized for release of N-glycans, O-glycans or both N- and O-glycans. An alternative method for the release of O-glycans is the LudgerLiberate ORELA Kit (LL-ORELA-A2).
The kit contains reagents and materials for up to 12 glycoprotein samples analysed in parallel or two sets of 6. Up to 1 mg of glycoprotein can be deglycosylated per sample.